WIPF2,WASP结合蛋白抗体

产品编号HZ-9869R
英文名称WIPF2
中文名称WASP结合蛋白抗体
别 名WAS/WASL interacting protein family member 2; WASP binding protein; WASP interacting protein related protein; WICH; WIP and CR16 homologous protein; WIP related protein; WIPF 2; WIR; WIPF2_HUMAN.
说 明 书0.1ml 0.2ml
研究领域心血管 信号转导 细胞骨架
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig,
WIPF2,WASP结合蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量46kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human WIPF2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
WIPF2,WASP结合蛋白抗体PubMedPubMed
产品介绍background:
WIRE, also known as WIPF2 (WAS/WASL interacting protein family, member 2) or WICH, is a 440 amino acid protein that localizes to both the cytoplasm and the cytoskeleton and contains one WH2 domain. Expressed ubiquitously with highest expression in colon, brain, lung and stomach, WIRE functions as an N-WASP-interacting protein that plays an important role in the organization and mobilization of the Actin cytoskeleton. Additionally, WIRE is involved in the formation of cell surface protrusions and may also provide a link between the cytoskeletal machinery and PDGF-B receptors. Multiple alternatively spliced isoforms of WIRE exist and are encoded by a gene that maps to human chromosome 17.

Function:
Plays an active role in the formation of cell surface protrusions downstream of activated PDGFB receptors. Plays an important role in actin-microspike formation through cooperation with WASL. May cooperate with WASP and WASL to induce mobilization and reorganization of the actin filament system.

Subunit:
Interacts with WASL and WASP, and this interaction results in cytoplasmic relocation of these two proteins along actin filaments. Interacts with NCK2 resulting in the localization to sites of focal adhesions. No interaction was seen with WASF2 and WASF3.

Subcellular Location:
Cytoplasm; cytoskeleton.

WIPF2,WASP结合蛋白抗体Similarity:
Belongs to the verprolin family.
Contains 1 WH2 domain.

Database links:
Entrez Gene: 147179 Human
Omim: 609692 Human
SwissProt: Q8TF74 Human
Unigene: 421622 Human
Unigene: 731598 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Paraformaldehyde-fixed, paraffin embedded (rat bain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WIPF2) Polyclonal Antibody, Unconjugated (HZ-9869R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining